Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia
Rev Bras Hematol Hemoter 2014;36:287-9 DOI: 10.1016/j.bjhh.2014.05.008
Case Report
Transmission of lupus anticoagulant by allogeneic stem cell transplantation
Mira Romany Massoud, Basem M. William??, , Katrina Harrill, Brenda Wimpfheimer Cooper, Marcos de Lima, Alvin H. Schmaier
University Hospitals Case Medical Center, Case Western Reserve University, Cleveland, United States
Received 28 November 2013, Accepted 09 December 2013
Abstract

Passive transmission of autoimmune diseases by allogeneic stem cell transplantation is rare and is ascribed to passive transfer of memory B-cells from donor to recipient. We hereby report a case of transmission of an asymptomatic lupus anticoagulant from a sibling donor to a recipient of transplantation for secondary acute myeloid leukemia. On pre-harvest evaluation, the sibling donor with no history of bleeding or thrombosis was found to have a lupus anticoagulant. After engraftment, the recipient was found to have a new prolonged activated partial thromboplastin time and was subsequently shown to have a lupus anticoagulant on Day +73 after stem cell transplantation. The recipient remained well with no evidence of bleeding, thrombosis, or graft-versus-host disease and was on a stable dose of tacrolimus at the time the lupus anticoagulant was detected. There was no other identifiable trigger for the appearance of a lupus anticoagulant.

Keywords
Allogeneic stem cell transplantation, Lupus anticoagulant, Activated partial thromboplastin time
Introduction

Allogeneic stem cell transplantation (SCT) is a potentially curative procedure for patients with hematological malignancies. Bone marrow and peripheral blood stem cells from related and unrelated donors are subject to a comprehensive evaluation to prevent the transmission of communicable diseases.1,2 Few cases illustrating the transmission of autoimmune disease by SCT have been reported in the literature. Such phenomena were attributed to the transmission of donor lymphocytes with SCT. The lupus anticoagulants (LAC) are heterogeneous antibodies that bind to a complex of anionic phospholipids that bind to one or more blood coagulation proteins to influence their contribution in coagulant-based assays.3 To our knowledge, there had not been any previously reported cases of the asymptomatic transmission of LAC by SCT. We hereby report a case of transmission of a LAC from a sibling donor to a recipient by SCT.

Case report

A 63-year-old Caucasian male, with no significant past medical history, was initially diagnosed with myelodysplastic syndrome with French, American and British (FAB) classification of refractory anemia with excess blasts; with complex cytogenetics and a revised international prognostic scoring system (IPSS-R) of 8.5.4 A bone marrow biopsy showed 6% blasts at the time of diagnosis. The patient received two cycles of azacitidine (75mg/m2 for seven doses). A repeat bone marrow biopsy showed transformation to secondary acute myeloid leukemia (AML) FAB subtype M6. The patient received induction chemotherapy with cytarabine (100mg/m2 for seven doses) and idarubicin (12mg/m2 for three doses) and achieved minimal residual disease with a markedly hypocellular marrow and no residual blasts. Given the high risk nature of his disease, the patient proceeded to allogeneic peripheral blood stem cell transplantation from his human leukocyte antigen- (HLA) and ABO-identical brother. The transplant conditioning regimen included fludarabine (daily dose of 30mg/m2 on Days −5 to −2) and melphalan (140mg/m2 on Day −2). Immunosuppression consisted of tacrolimus and methotrexate (10mg/m2 on Days +1, +3, +6, +11). Post-transplant course was complicated with Klebsiella pneumonia and vancomycin resistant enterococci bacteremia. The patient engrafted white cells on Day +15. On Day +73 the patient was doing well without evidence of graft-versus-host disease (GVHD), thrombosis, or bleeding. He had an isolated prolongation of activated partial thromboplastin time (aPTT) of 56s (normal range: 23–35). The patient's baseline value for aPTT prior to transplantation was 31s. The prothrombin time (PT) was 12.9s (normal range: 9.3–12.8). The thrombin time was normal excluding heparin contamination. Mixing studies failed to correct the prolonged aPTT. Dilute Russell Viper venom time (DRVVT) was 109.7s (normal range: <55.1s). DRVVT confirmatory test was 47.2s with a ratio of 2.2 (normal range: <1.3) demonstrating strong serologic evidence of a lupus anticoagulant (LAC). Serologic testing for anticardiolipin antibodies (IgG, IgM, IgA) and β2 glycoprotein I antibodies (IgG, IgM, IgA) was negative. The elevated PTT persisted. The patient's sibling donor was found to have a prolonged aPTT of 56s prior to donation. The screening test for DRVVT was 125.9; the confirmatory test was 41.7 with a ratio of 2.8 also indicating strong serologic evidence of LAC. These findings suggest the transmission of LAC from sibling donor to recipient.

Discussion

Autoimmune syndromes and autoantibodies are suspected to develop after autologous and allogeneic SCT. In a retrospective analysis of 1292 patients of post-transplantation (465 autologous and 827 allogeneic), 3% of patients developed a de novo LAC.5 The development of LAC in this analysis was related to the use of cyclosporine A, use of T-cell depletion for GVHD prophylaxis, use of a busulfan and cyclophosphamide (BUCY) regimen, and reactivation of cytomegalovirus (CMV) or other herpes viruses.5 Autoantibody production after SCT has been ascribed to de novo antibody production from naive B-cells or passive transfer of memory B-cells from the donor.6,7 B-cell functional recovery after SCT is slow and incomplete often resulting in seronegativity to previous immunizations; regardless of the immune status of the donor.8 Tracking of transferred B-cell clones showed that production of antigen-specific plasma cells from donor memory B-cell pool requires further exposure to antigen yet results in the production of antibodies with identical affinity to those produced in the donor.9

Antiphospholipid antibodies (aPL) are autoantibodies directed against phospholipid binding proteins that most commonly are β2-glycoprotein I, cardiolipin, and many blood coagulation proteins. LACs are aPL that interact with blood coagulation proteins and influence clot-based blood coagulation protein assays. The presence of lupus anticoagulants in healthy individuals has been estimated to be 1.0–5.6% while in individuals with systemic lupus erythematosus (SLE) it is much higher; 11–86%.10 The presence of LACs, in the absence of any history of bleeding or thrombosis, is frequently discovered by routine aPTT screening performed prior to an elective surgical procedure. The diagnosis of antiphospholipid syndrome (APLS) requires evidence of arterial or venous thrombosis or pregnancy morbidity associated with the detection of aPL antibodies on two separate occasions at least 12 weeks apart.3,10 In healthy individuals without SLE, the clinical significance of persistent asymptomatic aPL remains uncertain and the risk of development of thrombosis is unknown.10 APLS occurrence after SCT has been reported before but only in the context of acute/chronic GVHD and associated with cerebral thrombosis and catastrophic APLS.11–15

Ritchie et al. 13 described a case of the transmission of APLS from a sibling donor with known SLE, APLS, and history of thrombosis where the recipient developed symptomatic APLS with cerebral thrombosis and also a high titer anticardiolipin and anti-ds-DNA antibodies (identical to donor). In that report, aPL positivity was predated by the onset of chronic GVHD six months after SCT suggesting that memory B-cell activation occurred as the result of the presentation of phospholipid antigens from epithelial tissue induced by GVHD. In our case, we had prior knowledge that the sibling donor had LAC, yet we decided to proceed with SCT as we believed that the benefit in proceeding to SCT quickly, as the patient had a high risk for AML relapse, outweighed the theoretical risk of the development of symptomatic APLS. We cannot exclude the possibility of the development of a de novo autoimmune LAC in the recipient, independent of the transmission of donor lymphocytes capable of producing aPL (including LAC). Cell membrane damage, related to the toxicity from the conditioning regimen, may expose antigens, such as the hexagonal phase lipids, that are not otherwise present in the intact cell membrane. Such lipid could serve as an epitope for the development of LAC or other aPL as a de novo phenomenon.16 We believe that this possibility is less likely in the current case as the patient received a reduced-intensity conditioning regimen, had no evidence of GVHD, and was on a stable dose of tacrolimus at the time of the detection of the LAC. Our observations suggest the passive transfer of donor B-cells capable of producing a LAC. The antigen trigger for development of the LAC in our case remains obscure and may be related to infectious complications occurring in the peri-transplant period.

Conflicts of interest

The authors declare no conflicts of interest.

References
1
Banks AAoB
Standards for cellular therapy product services
2nd ed., American Association of Blood Banks, (2007)
2
EBMT FftAoCTaJACIa
FACT – JACIE international standards for cellular therapy product collection, processing, and administration
3rd ed., Foundation for the Accreditation of Cellular Therapy and Joint Accreditation Committee – ISCT and EBMT, (2006)
3
P.E. Love,S.A. Santoro
Antiphospholipid antibodies: anticardiolipin and the lupus anticoagulant in systemic lupus erythematosus (SLE) and in non-SLE disorders. Prevalence and clinical significance
Ann Intern Med, 112 (1990), pp. 682-698
4
M.T. Voso,S. Fenu,R. Latagliata,F. Buccisano,A. Piciocchi,M.A. Aloe-Spiriti
Revised International Prognostic Scoring System (IPSS-R) predicts survival and leukemic evolution of myelodysplastic syndromes significantly better than IPSS and who prognostic scoring system: validation by the gruppo romano mielodisplasie italian regional database
J. Clin. Oncol., 31 (2013), pp. 2671-2677 http://dx.doi.org/10.1200/JCO.2012.48.0764
5
E.W. Greeno,R. Haake,P. McGlave,D. Weisdorf,C. Verfaillie
Lupus inhibitors following bone marrow transplant
Bone Marrow Transplant, 15 (1995), pp. 287-291
6
J. Storek,A. Saxon
Reconstitution of B cell immunity following bone marrow transplantation
Bone Marrow Transplant, 9 (1992), pp. 395-408
7
E. Haddad,F. Le Deist,P. Aucouturier,M. Cavazzana-Calvo,S. Blanche,G. De Saint Basile
Long-term chimerism and B-cell function after bone marrow transplantation in patients with severe combined immunodeficiency with B cells: a single-center study of 22 patients
Blood, 94 (1999), pp. 2923-2930
8
D.C. Molrine,E.C. Guinan,J.H. Antin,C. Wheeler,S.K. Parsons,H.J. Weinstein
Haemophilus influenzae type b (HIB)-conjugate immunization before bone marrow harvest in autologous bone marrow transplantation
Bone Marrow Transplant, 17 (1996), pp. 1149-1155
9
B.F. Lausen,L. Hougs,L. Schejbel,C. Heilmann,T. Barington
Human memory B cells transferred by allogenic bone marrow transplantation contribute significantly to the antibody repertoire of the recipient
J Immunol, 172 (2004), pp. 3305-3318
10
A. Metjian,W. Lim
ASH evidence-based guidelines: should asymptomatic patients with antiphospholipid antibodies receive primary prophylaxis to prevent thrombosis?
Hematol Am Soc Hematol Educ Program, (2009), pp. 247-249
11
M.A. Kharfan-Dabaja,D. Morgensztern,E. Santos,M. Goodman,H.F. Fernandez
Acute graft-versus-host disease (aGVHD) presenting with an acquired lupus anticoagulant
Bone Marrow Transplant, 31 (2003), pp. 129-131 http://dx.doi.org/10.1038/sj.bmt.1703794
12
K.M. Kasamon,C.I. Drachenberg,A.P. Rapoport,A. Badros
Catastrophic antiphospholipid syndrome: atypical presentation in the setting of chronic graft versus host disease: case report and review of the literature
Haematologica, 90 (2005), pp. ECR17
13
D.S. Ritchie,A. Sainani,A. D'Souza,A.P. Grigg
Passive donor-to-recipient transfer of antiphospholipid syndrome following allogeneic stem-cell transplantation
Am J Hematol, 79 (2005), pp. 299-302 http://dx.doi.org/10.1002/ajh.20350
14
N. Sirachainan,S. Pakakasama,S. Hongeng,A. Chuansumrit,L. Tuntiyatorn,S. Vilaiyuk
Antiphospholid antibody syndrome and Hb E/Beta thalassemia disease post-allogeneic stem cell transplantation
Pediatr Blood Cancer, 57 (2011), pp. 153-156 http://dx.doi.org/10.1002/pbc.23020
15
D. Sohngen,A. Heyll,G. Meckenstock,C. Aul,H.H. Wolf,W. Schneider
Antiphospholipid syndrome complicating chronic graft-versus-host disease after allogeneic bone marrow transplantation
Am J Hematol, 47 (1994), pp. 143-144
16
J. Rauch,A.S. Janoff
Phospholipid in the hexagonal II phase is immunogenic: evidence for immunorecognition of nonbilayer lipid phases in vivo
Proc Natl Acad Sci U S A, 87 (1990), pp. 4112-4114
Corresponding author at: Division of Hematology and Hematology, University Hospitals Case Medical Center, 11100 Euclid Avenue, LKS 5079, Cleveland, OH 44106, United States. (Basem M. William bmw93@case.edu)
Copyright © 2014. Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular
Rev Bras Hematol Hemoter 2014;36:287-9 DOI: 10.1016/j.bjhh.2014.05.008