Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia
Rev Bras Hematol Hemoter 2017;39:108-14 DOI: 10.1016/j.bjhh.2016.09.017
Original article
Interleukin-10 haplotypes are not associated with acute cerebral ischemia or high-risk transcranial Doppler in a newborn cohort of 395 children with sickle cell anemia
André Rolim Belisárioa,b,, , Rahyssa Rodrigues Salesb, Nayara Evelin Toledoc, Cibele Velloso-Rodriguesd, Célia Maria Silvac, Marcos Borato Vianab
a Fundação Hemominas, Centro de Tecidos Biológicos de Minas Gerais, Lagoa Santa, MG, Brazil
b Universidade Federal de Minas Gerais (UFMG), Faculdade de Medicina, Belo Horizonte, MG, Brazil
c Fundação Hemominas, Serviço de Pesquisa, Belo Horizonte, MG, Brazil
d Universidade Federal de Juiz de Fora (UFJF), Governador Valadares, MG, Brazil
Recebido 14 Julho 2016, Aceitaram 22 Setembro 2016
Abstract
Background

The etiology of stroke, a severe complication of sickle cell anemia, involves inflammatory processes. However, the pathogenetic mechanisms are unknown. The aim of this study was to evaluate the influence of interleukin-10 polymorphisms and haplotypes on the risk of acute cerebral ischemia and high-risk transcranial Doppler in 395 children with sickle cell anemia from the state of Minas Gerais, Brazil.

Methods

Interleukin-10 haplotypes were determined by polymerase chain reaction-restriction fragment length polymorphism and sequencing. The outcomes studied were acute cerebral ischemia and high-risk transcranial Doppler. Clinical data were retrieved from the children's records.

Results

There was no statistically significant difference in the frequencies of polymorphisms and haplotypes between children with and without acute cerebral ischemia or children with or without high-risk transcranial Doppler. These data are consistent with a previous report that showed an absence of association between interleukin-10 plasma levels and high-risk transcranial Doppler velocity in children with sickle cell anemia.

Conclusion

Interleukin-10 haplotypes were not associated with the risk of acute cerebral ischemia or high-risk transcranial Doppler velocity in children with sickle cell anemia from the state of Minas Gerais, Brazil.

Keywords
Sickle cell anemia, Stroke, Polymorphism, Interleukin-10, Transcranial Doppler ultrasound
Rev Bras Hematol Hemoter 2017;39:108-14 DOI: 10.1016/j.bjhh.2016.09.017