Primary Cell Culture Media

Primary cell culture is defined as the ex vivo culture of cells freshly obtained from a tissue. The main advantages of primary cells are their faithful transcriptomic and proteomic profile and closer proximity to physiological function and response. Some drawbacks are their requirements for specific substrates and nutrients and the acquisition of a senescent phenotype, which leads to an irreversible cell cycle arrest that limits the amount of material that can be obtained. This prevents the use of primary cultures for large-scale disease modelling and drug discovery projects.