Phospho PKC θ (Y90) rabbit pAb

• Background :

  Catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., domain:The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain., enzyme regulation:Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation., function:PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters., function:This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR) -mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production., PTM:Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation., similarity:Belongs to the protein kinase superfamily., similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily., similarity:Contains 1 AGC-kinase C-terminal domain., similarity:Contains 1 C2 domain., similarity:Contains 1 protein kinase domain., similarity:Contains 2 phorbol-ester/DAG-type zinc fingers., subunit:Interacts with TXNL2/PICOT., tissue specificity:Skeletal muscle, megakaryoblastic cells and platelets.

• Description :

  Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Domain: The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. enzyme regulation: Three specific sites; Thr-538 (activation loop of the kinase domain), Ser-676 (turn motif) and Ser-695 (hydrophobic region), need to be phosphorylated for its full activation. function: PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. function: This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PTM: Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. similarity: Belongs to the protein kinase superfamily. similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. similarity: Contains 1 AGC-kinase C-terminal domain. similarity: Contains 1 C2 domain. similarity: Contains 1 protein kinase domain. similarity: Contains 2 phorbol-ester/DAG-type zinc fingers. subunit: Interacts with TXNL2/PICOT. tissue specificity: Skeletal muscle, megakaryoblastic cells and platelets.

• UniProt :

  Q04759

• Swiss Prot :

  Q04759

• Reactivity :

  Human; Mouse; Rat

• Immunogen :

  Synthesized peptide derived from human Phospho PKC θ (Y90)

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC

• Concentration :

  1 mg/ml

• Dilution :

  WB 1:500-2000; IHC-p 1:50-300

• Molecular Weight :

  78kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  78kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm. Cell membrane; Peripheral membrane protein. In resting T-cells, mostly localized in cytoplasm. In response to TCR stimulation, associates with lipid rafts and then localizes in the immunological synapse.

• Other Product Names :

  Protein kinase C theta type (EC 2.7.11.13; nPKC-theta)

• Gene ID (Human) :

  5588