Human S17aH (17-Alpha-Hydroxylase) ELISA Kit

• Product Name Alternative :

  Steroid 17-alpha-hydroxylase/17,20 lyase ;17-alpha-hydroxyprogesterone aldolase; CYPXVII; Cytochrome P450 17A1 ; Cytochrome P450-C17 ; Steroid 17-alpha-monooxygenaseCYP17; S17AH

• Reactivity :

  Human

• Field of Research :

  Enzyme & Kinase; Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Gastroenterology; Nutrition metabolism

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human S17aH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human S17aH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human S17aH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human S17aH in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  10 ng/mL

• Sample Type :

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  0.16-10 ng/mL

• Sensitivity :

  0.056 ng/mL

• Uniprot Accession Number :

  P05093