EasyStep Mouse NPT (Neopterin) ELISA Kit

• Product Name Alternative :

  Neopterin

• Reactivity :

  Mouse

• Field of Research :

  Cytokine; Infection immunity

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse NPT antigen, and the Mouse NPT standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse NPT to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse NPT in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  1.5h

• Standard :

  30 nmol/L

• Sample Type :

  Serum, plasma

• Detection Range :

  0.469-30 nmol/L

• Sensitivity :

  0.4 nmol/L