Mouse 8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit

• Product Name Alternative :

  8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine

• Reactivity :

  Mouse

• Field of Research :

  Metabolic pathway; Tumor immunity; Infection immunity; Endocrinology; Hematology; Hepatology

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 8-OHdG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  100 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  1.56-100 ng/mL

• Sensitivity :

  0.48 ng/mL