Mouse Anti-KLH IgM (Keyhole Limpet Hemocyanin IgM) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  Keyhole limpet IgM

• Reactivity :

  Mouse

• Field of Research :

  Immunology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Anti-KLH IgM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse Anti-KLH IgM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse Anti-KLH IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Anti-KLH IgM in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  100 ng/mL

• Sample Type :

  Serum, plasma, tissue homogenates

• Detection Range :

  1.57-100 ng/mL

• Sensitivity :

  0.51 ng/mL