Human AP1M1 (AP-1 Complex Subunit Mu-1) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  AP47; CLAPM2; CLTNM; MU-1A; mu1A; Adaptor Related Protein Complex 1 Subunit Mu 1; AP-mu chain family member mu1A

• UniProt :

  Q9BXS5

• Reactivity :

  Human

• Field of Research :

  Metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human AP1M1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AP1M1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AP1M1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  20 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  0.32-20 ng/mL

• Sensitivity :

  0.11 ng/mL