Simian Cys-C (Cystatin C) ELISA Kit

• Product Name Alternative :

  CST3; Cystatin 3; ARMD11; Post-Gamma-Globulin; Gamma Trace; Post-Gamma-Globulin; Neuroendocrine Basic Polypeptide; Amyloid Angiopathy And Cerebral Hemorrhage

• Reactivity :

  Simian

• Field of Research :

  Kidney biomarker; Urology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian Cys-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Cys-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian Cys-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5 h

• Standard :

  800 ng/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  12.5-800 ng/mL

• Sensitivity :

  5.8 ng/mL