TOK-1 rabbit pAb

• Background :

  This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

• Description :

  This gene product was isolated on the basis of its interaction with BRCA2 and p21 proteins. It is an evolutionarily conserved nuclear protein with multiple interacting domains. The N-terminal half shares moderate homology with regions of calmodulin and M-calpain, suggesting that it may also bind calcium. Functional studies indicate that this protein may be an important cofactor for BRCA2 in tumor suppression, and a modulator of CDK2 kinase activity via p21. This protein has also been implicated in the regulation of BRCA2 and RAD51 nuclear focus formation, double-strand break-induced homologous recombination, and cell cycle progression. Multiple transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008],

• Product Name Alternative :

  BCCIP; TOK1; BRCA2 and CDKN1A-interacting protein; P21- and CDK-associated protein 1; Protein TOK-1

• UniProt :

  Q9P287

• Swiss Prot :

  Q9P287

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  Synthesized peptide derived from TOK-1 . at AA range: 60-140

• Target :

  TOK-1

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  35kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  35kD

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasm, cytoskeleton, spindle pole . Colocalizes with BRCA2 in discrete nuclear foci (PubMed:15713648) . In interphase, preferential localizes to the mother centriole (PubMed:28394342) . Recruited to the spindle pole matrix and centrosome by microtubules and dynein/dynactin activity (PubMed:28394342) . .; [Isoform 1]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Isoform 1/beta tends to be less abundant at, and less strongly associated with, centrosomes than isoform 2/alpha. .; [Isoform 2]: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . Isoform 2/alpha tends to be more abundant

• Other Product Names :

  BCCIP; TOK1; BRCA2 and CDKN1A-interacting protein; P21- and CDK-associated protein 1; Protein TOK-1

• Gene ID (Human) :

  56647