ACAP1 (phospho Ser554) rabbit pAb

• Background :

  Domain:PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3) . May mediate ACAP1-binding to PIP2 or PIP3 containing membranes., enzyme regulation:GAP activity stimulated by phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidic acid., function:GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface., miscellaneous:Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes., PTM:Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration., similarity:Contains 1 Arf-GAP domain., similarity:Contains 1 BAR domain., similarity:Contains 1 PH domain., similarity:Contains 3 ANK repeats., subunit:Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1., tissue specificity:Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

• Description :

  Domain: PH domain binds phospholipids including phosphatidic acid, phosphatidylinositol 3-phosphate, phosphatidylinositol 3, 5-bisphosphate (PIP2) and phosphatidylinositol 3, 4, 5-trisphosphate (PIP3). May mediate ACAP1-binding to PIP2 or PIP3 containing membranes. enzyme regulation: GAP activity stimulated by phosphatidylinositol 4, 5-bisphosphate (PIP2) and phosphatidic acid. function: GTPase-activating protein (GAP) for ADP ribosylation factor 6 (ARF6) required for clathrin-dependent export of proteins from recycling endosomes to trans-Golgi network and cell surface. miscellaneous: Cells overexpressing ACAP1 show an accumulation of ITGB1 in recycling endosomes and inhibition of stimulation-dependent cell migration. Cells with reduced levels of ACAP1 or AKT1 and AKT2 show inhibition of stimulation-dependent cell migration. Cells overexpressing ACAP1 and PIP5K1C show formation of tubular structures derived from endosomal membranes. PTM: Phosphorylation at Ser-554 by PKB is required for interaction with ITGB1, export of ITGB1 from recycling endosomes to the cell surface and ITGB1-dependent cell migration. similarity: Contains 1 Arf-GAP domain. similarity: Contains 1 BAR domain. similarity: Contains 1 PH domain. similarity: Contains 3 ANK repeats. subunit: Interacts with GTP-bound ARF6. Interacts with third cytoplasmic loop of SLC2A4/GLUT4. Interacts with CLTC. Interacts with GULP1. Forms a complex with GDP-bound ARF6 and GULP1. tissue specificity: Highest level in lung and spleen. Low level in heart, kidney, liver and pancreas.

• UniProt :

  Q15027

• Swiss Prot :

  Q15027

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human Centaurin-beta1 around the phosphorylation site of Ser554. AA range:520-569

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Storage Conditions :

  -20°C/1 year

• Fragment :

  IgG

• Subcellular Location :

  Recycling endosome membrane ; Peripheral membrane protein ; Cytoplasmic side .

• Other Product Names :

  ACAP1; CENTB1; KIAA0050; Arf-GAP with coiled-coil; ANK repeat and PH domain-containing protein 1; Centaurin-beta-1; Cnt-b1

• Gene ID (Human) :

  9744