Human HVD3 (25-Hydroxyvitamin D3) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25 (OH) D

• Reactivity :

  Human

• Field of Research :

  Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  200 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  3.13-200 ng/mL

• Sensitivity :

  0.88 ng/mL