DHVD3 (1,25-Dihydroxyvitamin D3) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  DH-VD3; Calcitriol; Rocaltrol; Calcijex; 1,25- (OH) 2D3; 1,25-Dihydroxycholecalciferol; 1,25-Dihydroxy Vitamin D3

• Reactivity :

  General

• Field of Research :

  Metabolic pathway; Bone metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with DHVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to DHVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DHVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  10000 pg/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  156.25-10000 pg/mL

• Sensitivity :

  48.8 pg/mL