RT3 (Reverse Triiodothyronine) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  RT3; r-T3; Reverse T3; (2S) -2-Amino-3-[4- (4-Hydroxy-3,5-Diiodophenoxy) -3-Iodophenyl]propanoic Acid; 3,3',5'-Triiodo-L-thyronine

• Reactivity :

  General

• Field of Research :

  Infection immunity; Endocrinology; Hormone metabolism; Autoimmunity

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rT3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to rT3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of rT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  6000 pg/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  93.75-6000 pg/mL

• Sensitivity :

  27.68 pg/mL