Human HBg1 (Hemoglobin Gamma 1) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBg1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBg1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBg1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  HB-G1; HBGA; HBGR; HSGGL1; Gamma-1-globin

• UniProt :

  P69891

• Reactivity :

  Human

• Field of Research :

  Hematology

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBg1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBg1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBg1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  30000 ng/mL

• Sample Type :

  Serum, plasma, erythrocyte lysates

• Detection Range :

  468.75-30000 ng/mL

• Sensitivity :

  202.8 ng/mL