Rat UTS2R (Urotensin 2 Receptor) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat UTS2R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UTS2R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat UTS2R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UTS2R in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  UT; UTS2-R; GPR14; UTR2; Urotensin-II Receptor; Hypocretin Receptor; G Protein-Coupled Receptor 14

• UniProt :

  P49684

• Reactivity :

  Rat

• Field of Research :

  Endocrinology; Cardiovascular biology; Hormone metabolism

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat UTS2R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat UTS2R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat UTS2R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat UTS2R in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  20 ng/mL

• Sample Type :

  Tissue homogenates and other biological fluids.

• Detection Range :

  0.32-20 ng/mL

• Sensitivity :

  0.107 ng/mL