Human HMGB-2 (High Mobility Group Protein B2) ELISA Kit

• Product Name Alternative :

  High mobility group protein B2; HMGB2; HMG2; High mobility group protein 2

• Reactivity :

  Human

• Field of Research :

  Infection immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMGB-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMGB-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMGB-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMGB-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  5000 pg/mL

• Sample Type :

  Serum, plasma and other biological fluids

• Detection Range :

  78.13-5000 pg/mL

• Sensitivity :

  26.3 pg/mL

• Uniprot Accession Number :

  P26583