Human REST (RE1-Silencing Transcription Factor) ELISA Kit

• Product Name Alternative :

  RE1-silencing transcription factor; REST; NRSF

• Reactivity :

  Human

• Field of Research :

  Epigenetics and Nuclear Signaling; Developmental Biology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human REST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human REST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human REST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human REST in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  2000 pg/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  31.25-2000 pg/mL

• Sensitivity :

  11.2 pg/mL

• Uniprot Accession Number :

  Q13127