Mouse SYGP1 (Ras GTPase Activating Protein SynGAP) ELISA Kit

• Product Name Alternative :

  Ras/Rap GTPase-activating protein SynGAP; SYGP1; Syngap1; Neuronal RasGAP; Synaptic Ras GTPase-activating protein 1; Synaptic Ras-GAP 1

• Reactivity :

  Mouse

• Field of Research :

  Signal Transduction

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SYGP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SYGP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SYGP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SYGP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  5000 pg/mL

• Sample Type :

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  78.13-5000 pg/mL

• Sensitivity :

  24.1 pg/mL

• Uniprot Accession Number :

  F6SEU4