Human SLC5A8 (Solute Carrier Family 5, Member 8) ELISA Kit

• Product Name Alternative :

  Sodium-coupled monocarboxylate transporter 1; SLC5A8; Solute carrier family 5 member 8; AIT; SMCT ; SMCT1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter

• UniProt :

  Q8N695

• Reactivity :

  Human

• Field of Research :

  Signal transduction

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLC5A8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLC5A8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLC5A8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLC5A8 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  10 ng/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

• Detection Range :

  0.16-10 ng/mL

• Sensitivity :

  0.094 ng/mL