Recombinant K. pneumoniae NEO

• Background :

  Aminoglycoside 3'-phosphotransferase (APH (3') ), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH (3') is primarily found in certain species of gram-positive bacteria.

• Description :

  Recombinant Klebsiella Pneumoniae Aminoglycoside 3'-phosphotransferase is produced by our E.coli expression system and the target gene encoding Met1-Phe264 is expressed.

• UniProt :

  P00552

• Accession Number :

  P00552

• Endotoxin :

  Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

• Purity :

  Greater than 95% as determined by reducing SDS-PAGE.

• Molecular Weight :

  29 KDa

• Shipping Conditions :

  The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions :

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Storage Temperature :

  Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

• Notes :

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Observed Molecular Weight :

  26-30 KDa, reducing conditions

• Other Product Names :

  Aminoglycoside 3'-phosphotransferase; APH (3') -II; APH (3') II; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo