Recombinant Human OX40L (C-mFc)

• Background :

  Tumor necrosis factor ligand superfamily member 4 (TNFSF4/OX40L) is a single-pass type II membrane protein.OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40-OX40L co-stimulates signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. It involved in T-cell proliferation and cytokine production. Additional, it has been found association with systemic lupus erythematosus, no association with occurrence of atherosclerosis.

• Description :

  Recombinant Human OX40 Ligand is produced by our Mammalian expression system and the target gene encoding Gln51-Leu183 is expressed with a mFc tag at the C-terminus.

• UniProt :

  P23510

• Accession Number :

  P23510

• Endotoxin :

  Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

• Purity :

  Greater than 95% as determined by reducing SDS-PAGE.

• Bioactivity :

  Immobilized Human OX40-Fc (Cat#CK58) at 8μg/ml (100 μl/well) can bind Human OX40L-mFc (Cat#CW25) .The ED50 of Human OX40L-mFc (Cat#CW25) is 18.54 ng/ml.

• Molecular Weight :

  42.2 KDa

• Shipping Conditions :

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions :

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Storage Temperature :

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Notes :

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Observed Molecular Weight :

  50-70 KDa, reducing conditions

• Other Product Names :

  Tumor necrosis factor ligand superfamily member 4; Glycoprotein Gp34; OX40 ligand; OX40L; TAX transcriptionally-activated glycoprotein 1; TNFSF4; CD252; TXGP1