Recombinant Mouse B7-H4 (C-Fc)

• Background :

  Mouse V-set domain-containing T-cell activation inhibitor 1/VTCN1/B7-H4 is glycosylated member of the B7 family of immune co‐stimulatory proteins. B7-H4 consists of extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain. It is widely expressed, including in kidney, liver, lung, pancreas, placenta, prostate, spleen, testis and thymus. B7-H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. It also involved in promoting epithelial cell transformation.

• Description :

  Recombinant Mouse B7 Homolog 4 is produced by our Mammalian expression system and the target gene encoding Phe29-Pro258 is expressed with a Fc tag at the C-terminus.

• UniProt :

  AAH32925.1

• Accession Number :

  AAH32925.1

• Endotoxin :

  Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

• Purity :

  Greater than 95% as determined by reducing SDS-PAGE.

• Bioactivity :

  Immobilized Anti-Human B7-H4 mAb (Cat#NC054) at 2μg/ml (100 μl/well) can bind Mouse B7-H4-Fc* (Cat#CS04) .*: Biotinylated by NHS-biotin prior to testing The ED50 of Mouse B7-H4-Fc* (Cat#CS04) is 4.11 ng/ml.

• Molecular Weight :

  52.2 KDa

• Shipping Conditions :

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

• Storage Conditions :

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Storage Temperature :

  Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Notes :

  Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

• Observed Molecular Weight :

  70-90 KDa, reducing conditions

• Other Product Names :

  V-set domain containing T-cell activation inhibitor 1; B7 homolog 4; Protein B7S1; B7-H4; VTCN1