Plant γABA (Gamma-Aminobutyric Acid) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant γABA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant γABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant γABA in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  4-Aminobutyric Acid

• Reactivity :

  Plant

• Field of Research :

  Signal transduction; Cardiovascular biology; Neuro science; Bone metabolism

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Plant γABA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Plant γABA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Plant γABA in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2.5h

• Standard :

  2000 pg/mL

• Sample Type :

  Plant tissues

• Detection Range :

  31.25-2000 pg/mL

• Sensitivity :

  15.3 pg/mL