Rat MaR1 (Maresin 1) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MaR1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MaR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MaR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  7 (R) -Maresin 1; quinolin-7-ol; 7-hydroxychinoline; (4Z,7R,8E,10E,12Z,14S,16Z,19Z) -7,14-Dihydroxy-4,8,10,12,16,19-docosahexaenoic acid; 7-Chinolinol; 7R,14S-dihydroxydocosa-4Z,8E,10E,12Z,16Z,19Z-hexaenoic acid; 4,8,10,12,16,19-Docosahexaenoic acid, 7,14-dihydroxy-, (4Z,7R,8E,10E,12Z,14S,16Z,19Z) -; 7-Quinolinol; 7-HYDROXYQUINOLINE; 7R-maresin-1; 7-hydoxyquinoline

• Reactivity :

  Rat

• Field of Research :

  Immunology & Inflammation

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat MaR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MaR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MaR1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2.5h

• Standard :

  1000 pg/mL

• Sample Type :

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  15.63-1000 pg/mL

• Sensitivity :

  6.5 pg/mL