Human IL-37 (Interleukin 37) Microsample ELISA Kit

• Product Name Alternative :

  IL-1Z; IL1 Zeta; IL1F7; IL37; FIL1; FIL1 (ZETA) ; FIL1Z; IL-1F7; IL-1H4; IL-1RP1; IL1H4; IL1RP1; Interleukin 37; Interleukin-1-Related Protein; Interleukin 1 Family; Member 7

• UniProt :

  Q9NZH6

• Reactivity :

  Human

• Field of Research :

  Cytokine

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-37. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-37. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-37, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-37 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  500 pg/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  7.82-500 pg/mL

• Sensitivity :

  3.3 pg/mL