Human LAB7-2 (B-Lymphocyte Activation Antigen B7-2) Microsample ELISA Kit

• Product Name Alternative :

  CD86; B7-2; B70; FUN-1; CD28LG2; CD28 Antigen Ligand 2, B7-2 Antigen; CTLA-4 counter-receptor B7.2

• Reactivity :

  Human

• Field of Research :

  Signal transduction; CD & Adhesion molecule; Tumor immunity; Infection immunity; Immune molecule

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  10 ng/mL

• Sample Type :

  Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  0.16-10 ng/mL

• Sensitivity :

  0.056 ng/mL