Mouse 4-HNE (4-Hydroxynonenal) Microsample ELISA Kit

• Product Name Alternative :

  4-HNE, HNE, 4-hydroxy-2-nonenal, 6-Hydroxynonenal

• Reactivity :

  Mouse

• Field of Research :

  Signal transduction; Metabolic pathway; Infection immunity; Hormone metabolism; Dermatology

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse 4-HNE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse 4-HNE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse 4-HNE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse 4-HNE in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  150 ng/mL

• Sample Type :

  Serum, plasma and other biological fluids.

• Detection Range :

  2.35-150 ng/mL

• Sensitivity :

  1.1 ng/mL