Human F-C (Free Carnitine) ELISA Kit

• Description :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-C in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  Free Carnitine

• Reactivity :

  Human

• Field of Research :

  Signal transduction

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F-C. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F-C. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F-C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F-C in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5h

• Standard :

  400 nmol/mL

• Sample Type :

  Serum, plasma, tissue homogenates and other biological fluids

• Detection Range :

  6.25-400 nmol/mL

• Sensitivity :

  2.31 nmol/mL