THB (Tetrahydrobiopterin) ELISA Kit

• Product Name Alternative :

  Tetrahydrobiopterin; BH4; Kuvan; Sapropterin

• Reactivity :

  General

• Field of Research :

  Genetic diseases

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with THB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to THB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of THB in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  100 ng/mL

• Sample Type :

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  1.57-100 ng/mL

• Sensitivity :

  0.53 ng/mL