Simian TRACP-5b (TartSimiane Resistant Acid Phosphatase 5b) ELISA Kit

• Product Name Alternative :

  ACP5; TRAP5b; SPENCDI

• Reactivity :

  Simian

• Field of Research :

  Signal Transduction; Signal Transduction; Metabolism

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5 h

• Standard :

  50 ng/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  0.78-50 ng/mL

• Sensitivity :

  0.45 ng/mL