Simian VE-Cadherin (Vascular Endothelial Cadherin) ELISA Kit

• Product Name Alternative :

  CD144; 7B4; Cadherin 5 Type 2; VE-Cadherin; Cadherin, Vascular Endothelial; CDH5; Cadherin 5

• Reactivity :

  Simian

• Field of Research :

  CD & Adhesion molecule; Tumor immunity; Infection immunity

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian VE-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian VE-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian VE-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian VE-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5 h

• Standard :

  200 ng/mL

• Sample Type :

  Tissue homogenates, cell lysates and other biological fluids

• Detection Range :

  3.13-200 ng/mL

• Sensitivity :

  1.21 ng/mL