Simian RANTES (Regulated On Activation In Normal T-Cell Expressed And Secreted) ELISA Kit

• Product Name Alternative :

  CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A (Cys-Cys) Member 5

• Reactivity :

  Simian

• Field of Research :

  Cytokine; Tumor immunity; Infection immunity; Immune molecule

• Assay Type :

  Sandwich

• Assay Principle :

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian RANTES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian RANTES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian RANTES, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian RANTES in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  3.5 h

• Standard :

  5000 pg/mL

• Sample Type :

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range :

  78.13-5000 pg/mL

• Sensitivity :

  31.3 pg/mL