Chk2 (Phospho Thr432) rabbit pAb

• Background :

  Catalytic activity:ATP + a protein = ADP + a phosphoprotein., cofactor:Magnesium., disease:Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53., disease:Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]., disease:Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]., enzyme regulation:Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation., function:Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'., similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily., similarity:Contains 1 FHA domain., similarity:Contains 1 protein kinase domain., subcellular location:Isoform 10 is present throughout the cell., tissue specificity:High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

• Description :

  Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Disease: Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM: 609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. Disease: Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM: 259500]. Disease: Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM: 176807]. enzyme regulation: Rapidly phosphorylated on Thr-68 by MLTK in response to DNA damage and to replication block. Kinase activity is also up-regulated by autophosphorylation. function: Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. similarity: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. similarity: Contains 1 FHA domain. similarity: Contains 1 protein kinase domain. subcellular location: Isoform 10 is present throughout the cell. tissue specificity: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues.

• UniProt :

  O96017

• Swiss Prot :

  O96017

• Reactivity :

  Human; Mouse; Rat

• Immunogen :

  Synthesized peptide derived from human Chk2 (Phospho Thr432)

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  WB 1:1000-2000 ELISA 1:5000-20000

• Molecular Weight :

  60kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  60kD

• Fragment :

  IgG

• Subcellular Location :

  [Isoform 2]: Nucleus. Isoform 10 is present throughout the cell.; [Isoform 4]: Nucleus.; [Isoform 7]: Nucleus.; [Isoform 9]: Nucleus.; [Isoform 12]: Nucleus.; Nucleus, PML body. Nucleus, nucleoplasm. Recruited into PML bodies together with TP53.

• Other Product Names :

  Serine/threonine-protein kinase Chk2 (EC 2.7.11.1; CHK2 checkpoint homolog; Cds1 homolog; Hucds1; hCds1; Checkpoint kinase 2)

• Gene ID (Human) :

  11200