β-Tubulin Mouse mAb

• Background :

  Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

• Description :

  Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55, 000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of β-Tubulin may not be stable in certain cells. For example, expression of β-Tubulin in adipose tissue is very low and therefore β-Tubulin should not be used as loading control for these tissues.

• UniProt :

  P07437

• Swiss Prot :

  P07437

• Reactivity :

  Zebrafish

• Immunogen :

  Synthetic Peptide

• Clonality :

  Monoclonal

• Source :

  Mouse

• Applications :

  WB, IHC, IF

• Concentration :

  1mg/mL

• Dilution :

  WB 1:5,000-20,000 IHC 1:200 IF 1:200

• Buffer :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Molecular Weight :

  55kDa

• Storage Conditions :

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight :

  55kDa

• Fragment :

  IgG1

• Gene ID (Human) :

  203068