His-tag rabbit pAb

• Background :

  The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

• Description :

  The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

• Product Name Alternative :

  6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

• Reactivity :

  Species independent

• Immunogen :

  6X His synthetic peptide conjugated to KLH.

• Target :

  His-tag

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  15kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  15kD

• Fragment :

  IgG

• Other Product Names :

  6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag