RPA p32 (phospho Ser33) rabbit pAb

• Background :

  Function:Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions., PTM:Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis) . Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2., subcellular location:Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage., subunit:Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.

• Description :

  Function: Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. PTM: Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDC2. subcellular location: Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. subunit: Heterotrimer of 70, 32 and 14 kDa chains. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN.

• Product Name Alternative :

  RPA2; REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34

• UniProt :

  P15927

• Swiss Prot :

  P15927

• Reactivity :

  Human; Mouse; Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human RFA2 around the phosphorylation site of Ser33. AA range:1-50

• Target :

  RFA2

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC

• Concentration :

  1 mg/ml

• Dilution :

  WB 1:500-2000; IHC-p 1:50-300

• Buffer :

  -20°C/1 year

• Molecular Weight :

  32kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  32kD

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Nucleus, PML body . Redistributes to discrete nuclear foci upon DNA damage in an ATR-dependent manner. .

• Other Product Names :

  RPA2; REPA2; RPA32; RPA34; Replication protein A 32 kDa subunit; RP-A p32; Replication factor A protein 2; RF-A protein 2; Replication protein A 34 kDa subunit; RP-A p34

• Gene ID (Human) :

  6118