MEK Kinase-3 rabbit pAb

• Background :

  This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB) -dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

• Description :

  This gene product is a 626-amino acid polypeptide that is 96. 5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4. 6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

• UniProt :

  Q99759

• Swiss Prot :

  Q99759

• Reactivity :

  Human; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human MAP3K3. AA range:101-150

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

• Molecular Weight :

  71kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  71kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm, cytosol

• Other Product Names :

  MAP3K3; MAPKKK3; MEKK3; Mitogen-activated protein kinase kinase kinase 3; MAPK/ERK kinase kinase 3; MEK kinase 3; MEKK 3

• Gene ID (Human) :

  4215