P53RFP rabbit pAb

• Background :

  Caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family., domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme., function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism., pathway:Protein modification; protein ubiquitination., PTM:Auto-ubiquitinated., similarity:Belongs to the RBR family. RNF144 subfamily., similarity:Contains 1 IBR-type zinc finger., similarity:Contains 2 RING-type zinc fingers., subunit:Interacts with UBE2L3, UBE2L6 and LCMT2., tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.

• Description :

  Caution: Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family. Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. pathway: Protein modification; protein ubiquitination. PTM: Auto-ubiquitinated. similarity: Belongs to the RBR family. RNF144 subfamily. similarity: Contains 1 IBR-type zinc finger. similarity: Contains 2 RING-type zinc fingers. subunit: Interacts with UBE2L3, UBE2L6 and LCMT2. tissue specificity: Broadly expressed, with lowest levels in brain, spleen and thymus.

• Product Name Alternative :

  RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein

• UniProt :

  Q7Z419

• Swiss Prot :

  Q7Z419

• Reactivity :

  Human; Mouse

• Immunogen :

  Synthesized peptide derived from the Internal region of human p53RFP.

• Target :

  P53RFP

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  35kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  35kD

• Fragment :

  IgG

• Subcellular Location :

  Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm . Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.

• Other Product Names :

  RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein

• Gene ID (Human) :

  255488