Rad18 rabbit pAb

• Background :

  The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008]

• Description :

  The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens. [provided by RefSeq, Jul 2008],

• UniProt :

  Q9NS91

• Swiss Prot :

  Q9NS91

• Reactivity :

  Human; Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human RAD18. AA range:301-350

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC

• Concentration :

  1 mg/ml

• Dilution :

  WB 1:500-2000; IHC-p 1:50-300

• Molecular Weight :

  56kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  56kD

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Associates with chromatin (PubMed:25931565) . Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077) . Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607) . Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565) . .

• Other Product Names :

  RAD18; RNF73; E3 ubiquitin-protein ligase RAD18; Postreplication repair protein RAD18; hHR18; hRAD18; RING finger protein 73

• Gene ID (Human) :

  56852