PAOX rabbit pAb

• Background :

  Catalytic activity:N (1), N (12) -diacetylspermine + O (2) + H (2) O = N (1) -acetylspermidine + 3-acetamidobutanal + H (2) O (2) ., catalytic activity:N (1) -acetylspermidine + O (2) + H (2) O = putrescine + 3-acetamidopropanal + H (2) O (2) ., catalytic activity:N (1) -acetylspermine + O (2) + H (2) O = spermidine + 3-acetamidopropanal + H (2) O (2) ., cofactor:Binds 1 FAD per subunit., function:Flavoenzyme which catalyzes the oxidation of N (1) -acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N (1) -acetylspermidine to putrescine. Substrate specificity: N (1) -acetylspermine = N (1) -acetylspermidine > N (1), N (12) -diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs., induction:By polyamine analogs., miscellaneous:Oxidizes N (1) -acetylated polyamines on the exo-side of their N (4) -amino groups. Plant PAO oxidizes spermine on the endo-side of the N (4) -nitrogen., pathway:Amine and polyamine metabolism; spermine metabolism., similarity:Belongs to the flavin monoamine oxidase family., subunit:Monomer., tissue specificity:Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues.

• Description :

  Catalytic activity: N (1), N (12)-diacetylspermine + O (2) + H (2)O = N (1)-acetylspermidine + 3-acetamidobutanal + H (2)O (2). Catalytic activity: N (1)-acetylspermidine + O (2) + H (2)O = putrescine + 3-acetamidopropanal + H (2)O (2). Catalytic activity: N (1)-acetylspermine + O (2) + H (2)O = spermidine + 3-acetamidopropanal + H (2)O (2). Cofactor: Binds 1 FAD per subunit. function: Flavoenzyme which catalyzes the oxidation of N (1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N (1)-acetylspermidine to putrescine. Substrate specificity: N (1)-acetylspermine = N (1)-acetylspermidine > N (1), N (12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs. induction: By polyamine analogs. miscellaneous: Oxidizes N (1)-acetylated polyamines on the exo-side of their N (4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N (4)-nitrogen. pathway: Amine and polyamine metabolism; spermine metabolism. similarity: Belongs to the flavin monoamine oxidase family. subunit: Monomer. tissue specificity: Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues.

• UniProt :

  Q6QHF9

• Swiss Prot :

  Q6QHF9

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  Synthesized peptide derived from PAOX . at AA range: 260-340

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight :

  70kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  70kD

• Fragment :

  IgG

• Subcellular Location :

  Peroxisome . Cytoplasm .

• Other Product Names :

  PAOX; PAO; Peroxisomal N (1) -acetyl-spermine/spermidine oxidase; Polyamine oxidase

• Gene ID (Human) :

  196743