Apaf-1 rabbit pAb

• Background :

  This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC) . Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

• Description :

  This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding Cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

• Product Name Alternative :

  APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1

• UniProt :

  O14727

• Swiss Prot :

  O14727

• Reactivity :

  Human; Mouse; Rat

• Immunogen :

  The antiserum was produced against synthesized peptide derived from the Internal region of human APAF1. AA range:501-550

• Target :

  APAF1

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  135kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  135kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm .

• Other Product Names :

  APAF1; KIAA0413; Apoptotic protease-activating factor 1; APAF-1

• Gene ID (Human) :

  317