ERK 8 (phospho Thr175/Y177) rabbit pAb

• Background :

  Catalytic activity:ATP + a protein = ADP + a phosphoprotein., domain:The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation., domain:The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases., enzyme regulation:Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1., function:In vitro, phosphorylates MBP., PTM:Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro., PTM:Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase., similarity:Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily., similarity:Contains 1 protein kinase domain., subunit:Interacts with CSK/c-Src, ABL1, RET and TGFB1I1., tissue specificity:Widely expressed with a maximal expression in lung and kidney.

• Description :

  Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Domain: The N-terminal region (1-20) is the minimal region necessary for ubiquitination and further proteosomal degradation. Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. enzyme regulation: Activated by threonine and tyrosine phosphorylation. Inhibited by dual specificity phosphatases, such as DUSP1. function: In vitro, phosphorylates MBP. PTM: Dually phosphorylated on Thr-175 and Tyr-177, which activates the enzyme. Autophosphorylated on threonine and tyrosine residues in vitro. PTM: Ubiquitinated. Ubiquitination may allow its tight kinase activity regulation and rapid turnover. May be ubiquitinated by a SCF E3 ligase. similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. similarity: Contains 1 protein kinase domain. subunit: Interacts with CSK/c-Src, ABL1, RET and TGFB1I1. tissue specificity: Widely expressed with a maximal expression in lung and kidney.

• Product Name Alternative :

  MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8

• UniProt :

  Q8TD08

• Swiss Prot :

  Q8TD08

• Reactivity :

  Human; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human ERK8 around the phosphorylation site of Thr175 and Tyr177. AA range:141-190

• Target :

  ERK8

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  60kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  60kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm, cytoskeleton, cilium basal body . Cell junction, tight junction . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole . Cytoplasmic vesicle, autophagosome . Golgi apparatus . Nucleus . Cytoplasm . Cytoplasm, cytoskeleton, spindle . Co-localizes to the cytoplasm only in presence of ESRRA (PubMed:21190936) . Translocates to the nucleus upon activation (PubMed:20638370) . At prometaphase I, metaphase I (MI), anaphase I, telophase I, and metaphase II (MII) stages, is stably detected at the spindle (By similarity) . .

• Other Product Names :

  MAPK15; ERK7; ERK8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8

• Gene ID (Human) :

  225689