NFκB-p65 rabbit pAb

• Background :

  NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

• Description :

  NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011],

• UniProt :

  Q04206

• Swiss Prot :

  Q04206

• Reactivity :

  Human; Mouse; Rat; pig

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human NF-kappaB p65. AA range:247-296

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Molecular Weight :

  60kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  60kD

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Cytoplasm . Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B) (PubMed:1493333) . Colocalized with DDX1 in the nucleus upon TNF-alpha induction (PubMed:19058135) . Colocalizes with GFI1 in the nucleus after LPS stimulation (PubMed:20547752) . Translocation to the nucleus is impaired in L.monocytogenes infection (PubMed:20855622) . .

• Other Product Names :

  RELA; NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

• Gene ID (Human) :

  5970