Caspase-4 rabbit pAb

• Background :

  This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

• Description :

  This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008],

• UniProt :

  P49662

• Swiss Prot :

  P49662

• Reactivity :

  Human

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human Caspase-4. AA range:75-124

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

• Molecular Weight :

  45kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  45kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER) . Association with the ER membrane requires TMEM214 (PubMed:15123740) . Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630) . .

• Other Product Names :

  CASP4; ICH2; Caspase-4; CASP-4; ICE (rel) -II; Protease ICH-2; Protease TX

• Gene ID (Human) :

  837