Ksr-1 rabbit pAb

• Background :

  Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data., function:Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity., PTM:Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated., similarity:Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family., similarity:Contains 1 phorbol-ester/DAG-type zinc finger., similarity:Contains 1 protein kinase domain., subcellular location:In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery., subunit:Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity) . The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.

• Description :

  Caution: The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data. function: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity. PTM: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. similarity: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. similarity: Contains 1 phorbol-ester/DAG-type zinc finger. similarity: Contains 1 protein kinase domain. subcellular location: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. subunit: Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner (By similarity). The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.

• Product Name Alternative :

  KSR1; KSR; Kinase suppressor of Ras 1

• UniProt :

  Q8IVT5

• Swiss Prot :

  Q8IVT5

• Reactivity :

  Human; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human KSR. AA range:358-407

• Target :

  Ksr-1

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

• Buffer :

  -20°C/1 year

• Molecular Weight :

  115kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  115kD

• Fragment :

  IgG

• Subcellular Location :

  Cytoplasm . Membrane ; Peripheral membrane protein . Cell membrane ; Peripheral membrane protein . Cell projection, ruffle membrane . Endoplasmic reticulum membrane . In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery. .

• Other Product Names :

  KSR1; KSR; Kinase suppressor of Ras 1

• Gene ID (Human) :

  8844