MDC1 (phospho Ser513) rabbit pAb

• Background :

  The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008]

• Description :

  The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci. [provided by RefSeq, Jul 2008],

• UniProt :

  Q14676

• Swiss Prot :

  Q14676

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from human MDC1 around the phosphorylation site of Ser513. AA range:479-528

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  IHC; IF; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

• Storage Conditions :

  -20°C/1 year

• Fragment :

  IgG

• Subcellular Location :

  Nucleus . Chromosome . Associated with chromatin. Relocalizes to discrete nuclear foci following DNA damage, this requires 'Ser-139' phosphorylation of H2AX. Colocalizes with APTX at sites of DNA double-strand breaks.

• Other Product Names :

  MDC1; KIAA0170; NFBD1; Mediator of DNA damage checkpoint protein 1; Nuclear factor with BRCT domains 1

• Gene ID (Human) :

  9656