Perforin 1 rabbit pAb

• Background :

  The protein encoded by this gene has structural and functional similarities to complement component 9 (C9) . Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

• Description :

  The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008],

• Product Name Alternative :

  Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP)

• UniProt :

  P14222

• Swiss Prot :

  P14222

• Reactivity :

  Human; Rat; Mouse

• Immunogen :

  The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1. AA range:451-500

• Target :

  Perforin

• Clonality :

  Polyclonal

• Source :

  Rabbit

• Applications :

  WB; IHC; ELISA

• Concentration :

  1 mg/ml

• Dilution :

  WB 1:500-2000, IHC 1:50-200, ELISA 1:10000-20000

• Buffer :

  -20°C/1 year

• Molecular Weight :

  61kD

• Storage Conditions :

  -20°C/1 year

• Observed Molecular Weight :

  61kD

• Fragment :

  IgG

• Subcellular Location :

  Cytolytic granule . Secreted. Cell membrane ; Multi-pass membrane protein . Endosome lumen . Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell (PubMed:20038786) . Inserts into the cell membrane of target cells and forms pores (PubMed:20889983) . Membrane insertion and pore formation requires a major conformation change (PubMed:20889983) . May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786) . .

• Other Product Names :

  Perforin-1 (P1) (Cytolysin) (Lymphocyte pore-forming protein) (PFP)

• Gene ID (Human) :

  5551