Dog HbA1c (Glycated Hemoglobin A1c) ELISA Kit

• Description :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative :

  Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

• Reactivity :

  Dog

• Field of Research :

  Diagnosis of diabetes mellitus

• Assay Type :

  Competitive Inhibition

• Assay Principle :

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog HbA1c. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog HbA1c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog HbA1c in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time :

  2h

• Standard :

  1000 μg/mL

• Sample Type :

  Plasma and erythrocyte lysates

• Detection Range :

  15.63-1000 μg/mL

• Sensitivity :

  4.07 μg/mL